8Y9S

Crystal structure of nanobody MY6321 bound to human serum albumin (HSA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	65.260, 143.900, 93.670
Unit cell angles	90.00, 100.23, 90.00

Refinement procedure

Resolution	46.250 - 2.880
R-factor	0.23313
Rwork	0.230
R-free	0.29265
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.545
Data reduction software	XDS (0.7.7)
Data scaling software	XDS (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.250	2.950
High resolution limit [Å]	2.880	2.880
Rmerge	0.286	2.018
Rmeas	0.310	2.183
Rpim	0.120	0.825
Total number of observations		19122
Number of reflections	38264	2819
<I/σ(I)>	5.5	1.1
Completeness [%]	99.4
Redundancy	6.6	6.8
CC(1/2)	0.983	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.15 M DL-Malic acid pH 7.0, 20% w/v Polyethylene glycol 3,350