8Y9R

Crystal structure of Spiral2 microtubule-binding domain from Physcomitrella patens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97899
Spacegroup name	P 2 21 21
Unit cell lengths	59.826, 95.646, 150.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.095 - 2.799
Rwork	0.215
R-free	0.27640
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.856
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.150	1.593
Rmeas	0.163
Rpim	0.063	0.673
Total number of observations	161797
Number of reflections	24426	3148
<I/σ(I)>	10
Completeness [%]	99.6
Redundancy	6.6	6.5
CC(1/2)	0.998	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium citrate (pH 5.0), 0.2 M NaH2SO4, 50 mM guanidine hydrochloride and 1.4 M ammonium sulfate