8Y9A

The Crystal Structure of USP8 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.239850
Spacegroup name	H 3 2
Unit cell lengths	103.008, 103.008, 93.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.240 - 3.100
Rwork	0.198
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.240	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.119	0.824
Number of reflections	3507	640
<I/σ(I)>	9.6
Completeness [%]	98.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4.0M Na formate