8Y9A
The Crystal Structure of USP8 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.239850 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 103.008, 103.008, 93.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.240 - 3.100 |
| Rwork | 0.198 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.749 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.240 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.119 | 0.824 |
| Number of reflections | 3507 | 640 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4.0M Na formate |
