8Y97

Crystal structure of a heterooligomeric aminotransferase from Serratia sp. ATCC 39006, PMP-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-11-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.465, 180.934, 183.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.630 - 2.830
R-factor	0.22953
Rwork	0.228
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.630	2.940
High resolution limit [Å]	2.830	2.830
Rmerge	0.131	0.950
Rmeas	0.142	1.029
Rpim	0.055	0.392
Total number of observations		30199
Number of reflections	42789	4430
<I/σ(I)>	14.2	2.3
Completeness [%]	100.0
Redundancy	6.6	6.8
CC(1/2)	0.997	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	293	dipotassium hydrogenphosphate, sodium dihydrogenphosphate, lithium sulfate, CAPS