8Y96

Crystal structure of a heterooligomeric aminotransferase from Serratia sp. ATCC 39006

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-05-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.688, 181.029, 182.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.740 - 2.840
R-factor	0.20696
Rwork	0.204
R-free	0.25853
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.967
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	2.950
High resolution limit [Å]	2.840	2.840
Rmerge	0.117	0.773
Rmeas	0.127	0.839
Rpim	0.049	0.323
Total number of observations	279584	29180
Number of reflections	42411	4402
<I/σ(I)>	14.9	2.6
Completeness [%]	100.0
Redundancy	6.6	6.6
CC(1/2)	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	ammonium sulfate, MES