8Y75
Crystal structure of the CARF-HTH domain of Csx1-Crn2 from Marinitoga sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.884, 193.134, 61.213 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 38.600 - 2.400 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.080 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 47810 | 4749 |
| <I/σ(I)> | 14.68 | |
| Completeness [%] | 98.1 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.998 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Potassium nitrate pH 6.9, 20% PEG 3350 and 0.2 M Calcium acetate |
