8Y74
Crystal structure of 9-mer peptide from H9N2 avian influenza virus in complex with BF2*0201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-22 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.782, 76.553, 87.143 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 43.532 - 1.900 |
| Rwork | 0.204 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.645 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.532 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 65173 | 4776 |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 5.9 |
| CC(1/2) | 0.996 | 0.438 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000 |
