8Y6C
Norovirus GII.10 P domain and 2'-FL (powder)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95365 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.988, 78.700, 70.770 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 44.630 - 1.470 |
| R-factor | 0.1678 |
| Rwork | 0.167 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.630 | 1.490 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.067 | 1.313 |
| Rmeas | 0.075 | 1.494 |
| Rpim | 0.035 | 0.702 |
| Total number of observations | 556487 | 21864 |
| Number of reflections | 122545 | 5094 |
| <I/σ(I)> | 9.2 | 0.7 |
| Completeness [%] | 99.0 | |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.999 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.2 M sodium nitrate 0.1 M bis-tris propane (pH 7.5) 20% (w/v) PEG3350 |
