8Y68

the crystal structure of apo CASK-CaMK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	59.490, 93.680, 139.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.840 - 2.200
R-factor	0.2395
Rwork	0.238
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kmh
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.840	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	40508	2957
<I/σ(I)>	9.8
Completeness [%]	99.6
Redundancy	12.8
CC(1/2)	0.998	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Sodium citrate tribasic dihydrate, pH 5.0, 10% (v/v) PEG6000.