8Y5V
GII.4 Sydney PD and 2'-FL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.624, 55.763, 63.613 |
| Unit cell angles | 90.00, 120.14, 90.00 |
Refinement procedure
| Resolution | 41.690 - 1.540 |
| R-factor | 0.1561 |
| Rwork | 0.155 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.065 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.020 | 1.560 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.068 | 0.960 |
| Rmeas | 0.076 | 1.080 |
| Rpim | 0.034 | 0.489 |
| Total number of observations | 200633 | 8710 |
| Number of reflections | 42181 | 1890 |
| <I/σ(I)> | 11 | 1.1 |
| Completeness [%] | 95.3 | |
| Redundancy | 4.8 | 4.6 |
| CC(1/2) | 0.999 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | magnesium formate, 20% PEG 3350 |
