8Y4U

Crystal structure of a His1 from oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	I 2 2 2
Unit cell lengths	64.431, 101.028, 130.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.542 - 2.400
R-factor	0.2037
Rwork	0.201
R-free	0.24930
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.885
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.542	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	16984	1741
<I/σ(I)>	20.3
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.999	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	0.1M Hepes 25% PEG smear medium