8Y4B
Crystal structure of L-2-keto-3-deoxyfuconate 4-dehydrogenase bound to L-2,4-DKDF and NADH
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-10-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.868, 119.541, 129.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.320 - 1.670 |
| R-factor | 0.2688 |
| Rwork | 0.266 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.320 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 110122 | 5406 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.57 | |
| CC(1/2) | 0.991 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.2 M Sodium acetate, 0.1 M Tris-HCl pH8.5, 25% PEG4000 |
