8Y0L
beta-glucosidase from Thermoascus aurantiacus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 138.945, 149.752, 175.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.130 - 2.150 |
| R-factor | 0.16943 |
| Rwork | 0.168 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.921 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.207 |
| High resolution limit [Å] | 2.150 | 2.151 |
| Rmerge | 0.129 | 0.051 |
| Rmeas | 0.179 | |
| Number of reflections | 198186 | 13698 |
| <I/σ(I)> | 11.6 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH7.0, 0.14 M magnesium chloride, 20% 2-propanol, 0.5 mM 1-deoxynojirimycin |
