8Y0C
Crystal structure of FnCas12a in complex with pre-crRNA and 18nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.283, 124.283, 268.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.220 - 3.450 |
| R-factor | 0.2193 |
| Rwork | 0.216 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mga |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.510 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rpim | 0.102 | 0.279 |
| Number of reflections | 28418 | 1362 |
| <I/σ(I)> | 6 | 2.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 8.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M MES, pH 6.0, 0.15 M Sodium formate, 17% PEG 3350 |
