8Y09

Crystal structure of LbCas12a in complex with crRNA and 15nt target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	122.149, 143.401, 203.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 2.870
R-factor	0.2074
Rwork	0.204
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5XUZZ
RMSD bond length	0.011
RMSD bond angle	1.390
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rpim	0.100	0.402
Number of reflections	81224	4005
<I/σ(I)>	7.25
Completeness [%]	99.7	99.5
Redundancy	8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M Li2SO4, 30 mM Na2HPO4, 43.75 mM citric acid, 13% PEG 1000