8XY4
Crystal structure of VACV N1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.860, 77.860, 111.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.940 - 2.420 |
| Rwork | 0.170 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.660 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.940 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Number of reflections | 8116 | 826 |
| <I/σ(I)> | 24.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 35.7 | |
| CC(1/2) | 0.999 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.0, 20% w/v PEG 3350 |
