8XY3
Crystal structure of VARV P1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.064, 153.786, 132.146 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.160 - 2.200 |
| Rwork | 0.204 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8xy1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.150 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 31040 | 2655 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.993 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Calcium chloride dihydrate, 0.1 M Tris pH 8.0, 20% w/v PEG 6000 |
