8XX9
Rhodothermus marinus alpha-amylase RmGH13_47A CBM48-A-B-C domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.770, 102.389, 63.397 |
| Unit cell angles | 90.00, 96.42, 90.00 |
Refinement procedure
| Resolution | 43.500 - 1.550 |
| R-factor | 0.14972 |
| Rwork | 0.148 |
| R-free | 0.17529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.460 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.434 |
| Rpim | 0.021 | 0.212 |
| Number of reflections | 80052 | 3709 |
| <I/σ(I)> | 22.2 | 3.4 |
| Completeness [%] | 99.7 | 94.3 |
| Redundancy | 6.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES buffer, PEG 20000, PEG 4000, PEG 3350, sodium iodide |
