8XW5
Cryo-EM structure of the aspartate:alanine antiporter AspT mutant L60C
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.56 |
| Number of Particle | 1634276 |
| Software | crYOLO,RELION,cryoSPARC,SerialEM,Coot,PHENIX |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
