Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.516, 154.074, 100.781 |
| Unit cell angles | 90.00, 108.32, 90.00 |
Refinement procedure
| Resolution | 73.430 - 2.760 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.586 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.280 | 2.864 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.133 | |
| Number of reflections | 60804 | 6140 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6 | |
| CC(1/2) | 0.983 | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M ammonium acetate, 30% w/v polyethylene glycol 4000, 0.1 M sodium citrate tribasic dihydrate, pH 5.6 |
