8XUK
Structure of beta-1,2-glucanase from Photobacterium gaetbulicola (PgSGL3, ligand-free)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-26 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.284, 76.595, 94.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.213 - 1.200 |
| Rwork | 0.191 |
| R-free | 0.20800 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.940 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.213 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.096 | 0.433 |
| Rpim | 0.060 | 0.271 |
| Number of reflections | 114009 | 5320 |
| <I/σ(I)> | 11.6 | 4 |
| Completeness [%] | 96.0 | 91.5 |
| Redundancy | 6.4 | 6.6 |
| CC(1/2) | 0.993 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, MgCl2, PEG 3350 |
