8XUA

Outer membrane protein from Vibrio campbellii 25 kDa (OmpV25)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.99984
Spacegroup name	C 1 2 1
Unit cell lengths	114.540, 77.089, 143.062
Unit cell angles	90.00, 95.09, 90.00

Refinement procedure

Resolution	38.210 - 2.700
R-factor	0.2119
Rwork	0.209
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge		0.038	0.479
Rmeas	0.086	0.041	0.544
Rpim	0.032	0.015	0.249
Number of reflections	34012	1788	1549
<I/σ(I)>	6.4
Completeness [%]	99.1	99.4	89
Redundancy	7	7.2	4.1
CC(1/2)	0.786	0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	292	0.05 M Lithium phosphate 0.025 M Sodium acetate pH 5.0 23% v/v PEG 300