8XU5
The Crystal Structure of RORgT from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 99.809, 99.809, 125.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.256 - 3.500 |
Rwork | 0.201 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.181 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.370 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.177 | 1.076 |
Number of reflections | 9025 | 2156 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 99.9 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2M sodium formate, 3% MPD, 0.1M HEPES pH 7.4+ 0.1M Adenosine-5-triphosphate diNa salt |