8XT5

The closed state of RGLG5-VWA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	44.998, 66.945, 84.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.857 - 1.391
R-factor	0.1565
Rwork	0.155
R-free	0.18390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.369
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.857	1.420
High resolution limit [Å]	1.390	1.390
Rmeas	0.065
Number of reflections	51982	3108
<I/σ(I)>	1.36
Completeness [%]	99.6
Redundancy	12.5
CC(1/2)		0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	30% polyethylene glycol 8K, 0.5 M KCl, 0.1 M MES pH 6.5