8XS7
Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with FICZ
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.330, 98.514, 80.372 |
| Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
| Resolution | 34.164 - 2.770 |
| R-factor | 0.2108 |
| Rwork | 0.206 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.180 | 34.180 | 2.840 |
| High resolution limit [Å] | 2.770 | 12.390 | 2.770 |
| Rmerge | 0.144 | 0.032 | 0.733 |
| Number of reflections | 27183 | 317 | 1995 |
| <I/σ(I)> | 9.5 | 27.2 | 2.2 |
| Completeness [%] | 99.9 | ||
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | potassium citrate tribasic monohydrate, PEG3350 |
