8XRV
The crystal structure of a GH3 enzyme CcBgl3B with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 163.330, 177.574, 265.787 |
Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.400 |
R-factor | 0.1763 |
Rwork | 0.176 |
R-free | 0.21530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.970 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.124 | 0.684 |
Number of reflections | 288030 | 14283 |
<I/σ(I)> | 6.9 | |
Completeness [%] | 99.5 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG |