8XPV
The Crystal Structure of EphA2 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.931, 107.504, 40.691 |
| Unit cell angles | 90.00, 108.69, 90.00 |
Refinement procedure
| Resolution | 38.576 - 1.550 |
| Rwork | 0.164 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.576 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.087 | 0.844 |
| Number of reflections | 37926 | 1845 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 97.8 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium Acetate, 0.1M Sodium Cacodylate HCl pH6.5, 18% PEG 8000 |
