8XPG
The Crystal Structure of polo box domain of Plk4 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 62.304, 77.614, 120.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.700 |
| Rwork | 0.209 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.590 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.106 | 1.031 |
| Number of reflections | 16692 | 2170 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.00M Ammonium sulfate, 100mM TRIS, pH7.0, 200mM Lithium sulfate |
