8XPF

2OG-Fe(II) oxygenase-ColD in complex with collinodins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	121.363, 61.010, 56.346
Unit cell angles	90.00, 112.60, 90.00

Refinement procedure

Resolution	48.540 - 1.500
R-factor	0.1659
Rwork	0.165
R-free	0.18380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.826
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.580	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	58994	8822
<I/σ(I)>	13.7
Completeness [%]	96.5
Redundancy	4.1
CC(1/2)	0.997	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.01 M Cobalt (II) chloride hexahydrate, 0.1 M MES monohydrate pH 6.5, 1.8 M Ammonium sulfate