8XP5

The Crystal Structure of p53/BCL-xL fusion complex from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95370
Spacegroup name	P 1 21 1
Unit cell lengths	73.976, 68.437, 77.055
Unit cell angles	90.00, 112.36, 90.00

Refinement procedure

Resolution	49.360 - 2.550
Rwork	0.255
R-free	0.32760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.360	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.220	0.724
Number of reflections	20765	2602
<I/σ(I)>	4.1
Completeness [%]	88.9
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05M MgCl2, 0.1M MES pH6.5, 5% PEG 4000, 10% 2-Propanol, 30% w/v D-Sorbito