8XN6
The Crystal Structure of GSK3b from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.953725 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.694, 85.429, 178.091 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.751 - 2.400 |
Rwork | 0.192 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 1.209 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.750 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.086 | 1.039 |
Number of reflections | 50034 | 3441 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.8 | |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 5% Glycerol, 20mM MgCl2, 100mM Hepes pH 7.2 |