8XN6
The Crystal Structure of GSK3b from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953725 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.694, 85.429, 178.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.751 - 2.400 |
| Rwork | 0.192 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.209 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 1.039 |
| Number of reflections | 50034 | 3441 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 5% Glycerol, 20mM MgCl2, 100mM Hepes pH 7.2 |
