8XMS
Crystal structure of Porcine Circovirus type II Rep ATPase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-01-13 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.95915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.920, 103.930, 76.850 |
| Unit cell angles | 90.00, 109.90, 90.00 |
Refinement procedure
| Resolution | 59.330 - 2.200 |
| R-factor | 0.29073 |
| Rwork | 0.289 |
| R-free | 0.31926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.566 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.330 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 50956 | 4415 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M BIS-TRIS PH6.0, 0.2M Li2SO4, 22% w/v PEG 3350 |
