8XKE
The structure of HLA-A/14-3-D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.170, 67.250, 122.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 1.920 |
| R-factor | 0.2109 |
| Rwork | 0.209 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nqv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.005 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.470 | 61.070 |
| High resolution limit [Å] | 1.920 | 2.130 |
| Rmerge | 0.129 | |
| Rmeas | 0.134 | |
| Number of reflections | 34186 | 25142 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Bis-Tris, 20%w/v Polyethylene glycol monomethyl ether 5000 |
