8XJB
Crystal structure of AtNATA2 bound to Spermine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.730, 54.890, 51.910 |
| Unit cell angles | 90.00, 113.64, 90.00 |
Refinement procedure
| Resolution | 34.930 - 1.450 |
| R-factor | 0.154 |
| Rwork | 0.151 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1-4487-000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.930 | 1.500 |
| High resolution limit [Å] | 1.448 | 1.450 |
| Number of reflections | 33619 | 3139 |
| <I/σ(I)> | 13.91 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.470 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M Ammonium sulfate, 0.1 M Tris pH 8.5, and 25% w/v Polyethylene glycol 3,350 |
