8XJ9

Crystal Structure of Arabidopsis N-amino acetyltransferase 2 (AtNATA2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	73.700, 54.840, 51.700
Unit cell angles	90.00, 113.90, 90.00

Refinement procedure

Resolution	36.640 - 1.100
R-factor	0.1541
Rwork	0.153
R-free	0.19549
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.796
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.270	1.120
High resolution limit [Å]	1.100	1.100
Number of reflections	75934	3489
<I/σ(I)>	8.3	0.8
Completeness [%]	99.5
Redundancy	6.7
CC(1/2)	0.990	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3,350