8XI7
The Crystal Structure of UCHL1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.216, 109.216, 79.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.840 - 1.950 |
| Rwork | 0.194 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | 1.056 |
| Number of reflections | 35583 | 2445 |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.1M ammonium sulfate, 2% PEG 400, 100mM HEPES, pH 7.0 |
