8XG4

X-ray crystal structure of streptavidin flash-cooled in 30% glycerol at ambient pressure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-20
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	46.739, 85.686, 58.228
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	46.164 - 1.380
R-factor	0.1376
Rwork	0.136
R-free	0.16930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.750	1.400
High resolution limit [Å]	1.380	1.380
Rmerge	0.046	0.816
Number of reflections	92788	4543
<I/σ(I)>	14.4	1.5
Completeness [%]	99.9	100
Redundancy	4.2	4.2
CC(1/2)	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH 6.0, 20% PEG1,000