8XFZ
The structure of HLA-A/L1-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.490, 70.826, 117.044 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.240 - 2.320 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.25070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.875 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.250 | 2.400 |
High resolution limit [Å] | 2.320 | 2.320 |
Rpim | 0.029 | 0.029 |
Number of reflections | 21060 | 21060 |
<I/σ(I)> | 18 | |
Completeness [%] | 100.0 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Imidazole, 20%w/v PEG 6000 |