8XFM

The Crystal Structure of MNK2 from Biortus.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	103.859, 103.859, 73.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.013 - 2.600
Rwork	0.190
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.226
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.013	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.081	0.870
Number of reflections	14349	968
<I/σ(I)>	27.5	3.5
Completeness [%]	100.0
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NH4Ac, 0.1M Bis-Tris pH5.5, 25% PEG 3350