8XFL
The Crystal Structure of MARK4 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.447, 78.103, 86.155 |
| Unit cell angles | 90.00, 122.00, 90.00 |
Refinement procedure
| Resolution | 41.697 - 3.000 |
| Rwork | 0.207 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.941 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.530 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.074 | 0.727 |
| Number of reflections | 13003 | 912 |
| <I/σ(I)> | 15.1 | 2.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Potassium sodium tartrate, 20% PEG 3350 |
