8XF2
Crystal structure of the reassembled C-phycocyanin hexamer from Thermoleptolyngbya sp. O-77
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 107.729, 115.069, 186.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 1.980 |
R-factor | 0.1832 |
Rwork | 0.183 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 1.222 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 162251 | 25284 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.9 | |
Redundancy | 7 | |
CC(1/2) | 0.991 | 0.435 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8% Tacsimate (pH 5.0), 20% polyethylene glycol 3350 |