8XDO
O-methyltransferase from Lycoris longituba complexed with Mg and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.04500 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.637, 81.183, 118.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.730 - 1.370 |
| R-factor | 0.1461 |
| Rwork | 0.145 |
| R-free | 0.16830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.089 | 1.249 |
| Number of reflections | 193630 | 4947 |
| <I/σ(I)> | 16.9 | 2.2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 13.5 | 13 |
| CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 21 % (w/v) PEG 8000, 0.2M MgCl2, 0.1M Tris-HCl (pH 8.0) |
