8XCU

Cryo-EM structure of Glutamate dehydrogenase from Thermococcus profundus incorporating NADPH, AKG and GLU in the steady stage of reaction

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	JEOL CRYO ARM 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.83
Number of Particle	6334528
Software	RELION,SerialEM,CTFFIND,Coot,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION