8XBD
GH18 family chitinase from cold seep metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-21 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54184 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 69.278, 114.286, 110.478 |
| Unit cell angles | 90.00, 93.20, 90.00 |
Refinement procedure
| Resolution | 19.090 - 2.400 |
| R-factor | 0.2023 |
| Rwork | 0.200 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.947 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.090 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.530 |
| Rmeas | 0.144 | 0.648 |
| Rpim | 0.059 | 0.366 |
| Total number of observations | 181837 | 10362 |
| Number of reflections | 33470 | 3509 |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 99.6 | |
| Redundancy | 5.4 | 3 |
| CC(1/2) | 0.992 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M potassium bromide, 30% (w/v) polyethylene glycol monomethyl ether 2,000 |
