8XBB

The substrate binding protein of an ABC transporter in complex with beta-1,3-xylobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	61.350, 74.379, 85.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.800 - 1.570
R-factor	0.1875
Rwork	0.186
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	1.660
High resolution limit [Å]	1.570	1.570
Number of reflections	54966	7901
<I/σ(I)>	9.1
Completeness [%]	99.9
Redundancy	8.2
CC(1/2)	0.995	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	1M lithium chloride, 0.1M citric acid (pH 4.0), 20% (w/v) polyethylene glycol (PEG) 6000