8XAP

Thermostable and acid-tolerant DNA-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-22
Detector	RAYONIX MX300HE
Wavelength(s)	0.99984
Spacegroup name	C 2 2 21
Unit cell lengths	48.253, 68.922, 173.558
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.610 - 1.700
R-factor	0.209
Rwork	0.207
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.056	0.439
Rpim	0.026	0.200
Number of reflections	32123	3197
<I/σ(I)>	35.2
Completeness [%]	99.0
Redundancy	5.8
CC(1/2)	0.965	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M sodium acetate, pH 4.6, 2.0M sodium formate