8XAO

The thermostable and acid-tolerant DNA-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-22
Detector	RAYONIX MX300HE
Wavelength(s)	0.99984
Spacegroup name	P 65 2 2
Unit cell lengths	81.892, 81.892, 139.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.190 - 2.050
R-factor	0.2035
Rwork	0.202
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.050	0.575
Rpim	0.017	0.200
Number of reflections	17976	1734
<I/σ(I)>	39.8
Completeness [%]	99.9
Redundancy	9.3
CC(1/2)	0.983	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M sodium acetate pH 5.5, 0.2M KBr, 25% PEG 2000 MME