8XAK
Structure of Pif1-G4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.163, 125.141, 258.233 |
Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
Resolution | 48.000 - 3.500 |
R-factor | 0.2859 |
Rwork | 0.282 |
R-free | 0.32350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.061 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 3.625 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 22444 | 2168 |
<I/σ(I)> | 3.05 | |
Completeness [%] | 99.3 | |
Redundancy | 1.9 | |
CC(1/2) | 0.904 | 0.611 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.9, 22.5 %(w/v) PEG 400, 0.1 M sodium acetate |