8X9V

Crystal structure of Xanthomonas oryzae pv. oryzae NADH-quinone oxidoreductase subunits NuoE and NuoF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2022-09-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	92.200, 99.910, 147.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 2.360
Rwork	0.192
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q9c
RMSD bond length	0.009
RMSD bond angle	1.065
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	2.420
High resolution limit [Å]	2.360	2.360
Number of reflections	56860	53377
<I/σ(I)>	14.6
Completeness [%]	99.9
Redundancy	13.3
CC(1/2)	0.999	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	Bis-Tris, PEG 4000, acetone