8X7Z

Crystal structure of CCoV-HuPn-2018 fusion core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2022-11-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	45.615, 45.615, 428.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.450 - 2.100
R-factor	0.2519
Rwork	0.251
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.474
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.450	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	10736	10736
<I/σ(I)>	2
Completeness [%]	99.7
Redundancy	1
CC(1/2)	0.800	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M TRIS pH 8.5, 25% PEG 4000